|
microarray TECHNOLOGY |
expression DATA |
data ANALYSIS |
model BUILDING |
selected PUBLICATIONS |
DNA chip technologies are distinguished by (a) sized of the arrayed DNA fragments, (b) the methods of arraying, (c) the chemistries and linkers for immobalizing DNA to a chip, and (d) the hybridization and detection techniques. Besides material extracted from cell populations, a procedure SCOMPTM has been reported (C.A. Klein et al. Proc. Natl. Acad. Sci. USA 96 4494-4499), which allows for single cell comparitive genomic hybridization.
DNA chip TECHNOLOGIES (sources: TIBS 24: MAY 1999,...)
| INSTITUTIONS | TECHNOLOGY |
|
Oligonucleotide formats
Affymetrix, Santa Clara, CA, USA |
High-density synthesis of oligonucleotides (oligos) in situ by photolitography |
| Protogene Laboraties, Palo Alto, CA, USA | Robotic serial synthesis of 50-mer oligos by piezoelectric pulse `ink jet' delivery |
| Englehardt Institute of Molecular Biology, Moscow, Russia | Arraying oligos attached to acrylamide pads on glass |
| Nanogen, San Diego, CA, USA | Accelerated hybridization using electrical fields |
| Baylor College of Medicine, HOuston, TX, USA | Mutation detection by solid-phase primer extension |
|
Sequenom, San Diego, CA, USA BRAX, Cambridge, UK GeneTrace Systems, Menlo Park, CA, USA |
Characterization of hybridizing DNA using mass spectrometry . . |
| Hyseq, Sunnyval, CA, USA | High-thruput array technology using flexible membranes |
|
cDNA formats Pat Brown Laboratory, Stanford University, Stanford, CA, USA |
Comprehensive reference site for building a capillary-arraying robot and scanner, and protocols for arraying and hybridization |
| Department Hans Lehrach, Max-Planck-Institute for Genetics, Berlin-Dahlem, Germany | High-speed arraying of colony/DNA samples on (22cm)2 nylon membranes (52 patterns containin a central ink guide dot surrounded by twelve clones spotted in duplicate, 57,600 clones) by spotting robots, carrying a 384 pin head and allowing spotting densities of ~300 spots/cm2, guide dots permit automatic grid finding and image analysis |
|
Amersham Pharmacia Biotech, Molecular Dynamics,
Sunnyvale, CA, USA TeleChem International, San Jose, VA, USA General Scanning Inc., Watertown, MA, USA Genomic Solutions, Ann Arbor, MI, USA Genetic Microsystems, Woburn, MA, USA Genomic Instrumentation Services, Menlo Park, CA, USA Fraunhofer Institute of Physical Measurement Technique IPM, Freiburg, Germany DymlerChrysler Aerospace Jena-Optronik GmbH DJO, Jena, Germany |
Commercial sources for arraying-robots and/or DNA chip scanners . . . . . . . |
| CLONTECH Laboratories, Palo Alto, CA, USA | ATLASTM human cancer/mouse cDNA expression array |
|
Reverse trancriptase polymerase chain reaction Incyte, Synteni Technology, CA, USA |
Gene Expression Matrix GEMTM for analysis of differential expression in normal versus diseased/treated cells, screen of two poly-(A) RNA samples per microarray in a competitive hybridization, and data analysis on the results |
|
Serial analysis of gene expression Genzyme Molecular Oncology GMO, Framingham, MA, USA |
SAGETM simultaneous detection of levels expressed genes in a wide variety of applications to ID disease-related genes |
| Leiden/A'dam Center for Drug Research, Leiden, The Netherlands | microSAGE, modified SAGE in limited amounts of tissue |
|
Fast array surface slides |
|
|
Bead-based Fiber-optic Arrays |
|
|
Protein 2D arrays |
|
| backhome |
Would you bet a hundred dollars against a dollar
that parity is not violated?, he asked.
No. But fifty dollars I will. -R.P. Feynman