HuSiDa
Human siRNA
Database
Instructions
i)
Searching the database
ii) Output
iii) Rearranging the output table
i) Searching the database
Each database record is composed of the target gene name
including the corresponding gene accession numbers (UniGene, RefSeq), the
siRNA sequence (sense strand), its activity in the percentage of gene
silencing (if available), manuscript reference (PubMed), the cell line and
transfection method used, the siRNA source and the target gene description.
To retrieve functional siRNAs sequences for a specific gene, the human siRNA
database can be searched for UniGene cluster IDs, gene names, RefSeq
accession numbers and target gene descriptions.
For example siRNA sequences targeting MDM2 can be identified by
searching for:
1.) the UniGene
code
Hs.212217 in the UniGene search field
2.) MDM2 in the gene
name field
3.) the RefSeq accession
number
NM_002392.2
4.) MDM2
in the target gene description field
To retrieve transfection methods for a particular cell line, the name of the cell line has to be entered into the corresponding field of the search mask. A database search with "HUVEC" (Human Umbilical Vein Endothelial Cells) yields several entries refer to publications that describe siRNA delivery into HUVEC cells by transfection with Oligofectamine or TransGeneII and electroporation.
ii) Output
The HuSiDa serach results are displayed as a table that can be
rearranged by clicking the icons above the columns.
iii)
Rearranging the output table
The output table can be rearranged by sorting coulmns up and down, hiding
columns and moving columns to the left or to the right trough clicking one
of the following icons:
sorts a column
up
sorts a column down
hides a column
moves a column to the left
moves a column to the rigt
restores original table
contact: Matthias.Truss@Charite.de